Separating cells / Dipak Patel.
Series: Basics (BIOS Scientific Publishers)Publication details: Oxford : BIOS ; New York : Springer, c2001.Description: vii, 168 p. : ill. ; 25 cmISBN:- 0387916121
- 1859961487
- 571.6078 21 PAT
- 2001 A-757
- QH 585.5.C44
Item type | Current library | Call number | Copy number | Status | Date due | Barcode |
---|---|---|---|---|---|---|
Book Closed Access | Health Sciences Library | 571.6078 PAT 1 (Browse shelf(Opens below)) | 1 | Available | MBAL22065563 |
Contents
CHAPTER 1. PREPARATION OF CELL SUSPENSIONS.
1.Diversity of cells.
2.Why separate living cells?.
3.Preparation of suspensions of single cells.
4.Viability measurements.
5.Separation of viable and nonviable cells.
6.Characterization of cells.
CHAPTER 2. FRACTIONATION OF CELLS BY SEDIMENTATION METHODS.
1.Introduction.
2.Separation media.
3.Iso-osmotic gradients.
4.Choice of separation method.
5.Separation of cells on the basis of size.
6.Separation of cells on the basis of their density.
7.Concluding remarks.
CHAPTER 3. CENTRIFUGAL ELUTRIATION.
1.Introduction.
2.Principles.
3.Equipment.
4.Conditions affecting separation.
5.Applications of centrifugal elutriation.
6.Advantages and disadvantages
CHAPTER 4. FREE-FLOW ELECTROPHORESIS.
1.Introduction.
2.Main types of FFE equipment.
3.Buffers.
4.Preparation of cell samples for FFE.
5.FFE of cell samples.
6.Factors affecting FFE.
7.Applications of FFE.
CHAPTER 5. AFFINITY SEPARATION.
1.Introduction.
2.Separation by nonmagnetic techniques.
3.Separation by magnetic techniques.
CHAPTER 6. FLOW CYTOMETRY.
1.Introduction.
2.The flow cytometer-instrumentation.
3.Types of flow sorting.
4.Factors affecting cell sorting.
5.Separating-purity, yield and sterility.
6.Applications.
List of manufacturers and Suppliers.
Includes bibliographical references and index.
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