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Nitric oxide protocols / edited by Michael A. Titheradge.

Contributor(s): Series: Methods in molecular biology (Clifton, N.J.) ; v. 100.Publication details: Totowa, N.J. : Humana Press, c1998.Description: xi, 324 p. : ill ; 24 cmISBN:
  • 0896035379
  • 9780896035379
Subject(s): LOC classification:
  • QV 126 N731 1998
Contents:
Contents 1.Enzymology of Nitric Oxide Synthases 2.Purification of the Inducible Nitric Oxide Synthase 3.Purification of the Constitutive Nitric Oxide Synthase 4.Cloning and Expression of Human Inducible Nitric Oxide Synthase 5.Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System 6.Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO 7.Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation 8.Radiochemical Measurement of NOS Activity by Conversion of [14C]L-Arginine to Citrulline Using HPLC Separation 9.The Enzymatic Measurement of Nitrate and Nitrite 10.Determination of NOS Activity Using Cyclic-GMP Formation 11.Determination of NO with a Clark-Type Electrode 12.The Measurement of NO in Biological Systems Using Chemiluminescence 13.Measurement of NO Using Electron Paramagnetic Resonance 14.Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction 15.Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay 16.Detection of NOS Isoforms by Western-Blot Analysis 17.Immunohistochemical Localization of NOS Isoforms 18.A Practical Protocol for the Demonstration of NOS Using In-Situ Hybridization 19.Use of NO Donors in Biological Systems 20.Making and Working with Peroxynitrite 21.The Use of NO Gas in Biological Systems 22.A Microtiter-Plate Assay of Human NOS Isoforms 23.Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings 24.Measurement of Biopterin and the Use of Inhibitors of Biopterin Biosynthesis 25.A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase I 26.Simultaneous Measurement of Mitochondrial Function and NO 27.Release of NO from Donor Compounds: A Mathematical Model for Calculation of NO Concentrations in the Presence of Oxygen 28.The Determination of Nitrotyrosine Residues in Proteins 29.Measurement of DNA Damage Using the Comet Assay 30.Methods for the Study of NO-Induced Apoptosis in Cultured Cells.
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Item type Current library Call number Copy number Status Date due Barcode
Book Open Access Book Open Access Health Sciences Library QV 126 N731 1998 (Browse shelf(Opens below)) 1 Available MBAL22020575

Includes bibliographical references and index.

Contents

1.Enzymology of Nitric Oxide Synthases
2.Purification of the Inducible Nitric Oxide Synthase
3.Purification of the Constitutive Nitric Oxide Synthase
4.Cloning and Expression of Human Inducible Nitric Oxide Synthase
5.Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System
6.Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO
7.Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation
8.Radiochemical Measurement of NOS Activity by Conversion of [14C]L-Arginine to Citrulline Using HPLC Separation
9.The Enzymatic Measurement of Nitrate and Nitrite
10.Determination of NOS Activity Using Cyclic-GMP Formation
11.Determination of NO with a Clark-Type Electrode
12.The Measurement of NO in Biological Systems Using Chemiluminescence
13.Measurement of NO Using Electron Paramagnetic Resonance
14.Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction
15.Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay
16.Detection of NOS Isoforms by Western-Blot Analysis
17.Immunohistochemical Localization of NOS Isoforms
18.A Practical Protocol for the Demonstration of NOS Using In-Situ Hybridization
19.Use of NO Donors in Biological Systems
20.Making and Working with Peroxynitrite
21.The Use of NO Gas in Biological Systems
22.A Microtiter-Plate Assay of Human NOS Isoforms
23.Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings
24.Measurement of Biopterin and the Use of Inhibitors of Biopterin Biosynthesis
25.A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase I
26.Simultaneous Measurement of Mitochondrial Function and NO
27.Release of NO from Donor Compounds: A Mathematical Model for Calculation of NO Concentrations in the Presence of Oxygen
28.The Determination of Nitrotyrosine Residues in Proteins
29.Measurement of DNA Damage Using the Comet Assay
30.Methods for the Study of NO-Induced Apoptosis in Cultured Cells.

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