The laboratory zebrafish / Claudia Harper, Christian Lawrence.

Contents

1 Biology
introduction
natural history
genetics
genetic strains
nomenclature
behavior
anatomy and physiology
Integumentary System
Musculoskeletal System
Gastrointestinal System
Respiratory System
Swim Bladder
Genitourinary System
Cardiovascular System
Hematopoietic and Lymphoid System
Endocrine System
Pituitary gland
Adrenal gland
Thyroid gland
Parathyroid glands
Pancreas
Ultimobranchial gland
Corpuscles of Stannius
Urophysis
Pineal gland
Nervous and Sensory Systems
clinical chemistry and hematology
Blood Volume
Hematology

2 Husbandry
nutrition and feeding
Nutrient Requirements
Proteins/amino acids
Lipids
Carbohydrates
Vitamins
Minerals
Feed Types
Live feeds
Processed feeds
Life Stages
Larvae
Juveniles
Adults
breeding
Reproductive Cycle
Controlling Factors
Reproductive Behavior
Spawning Techniques and Equipment
In-tank strategies
Static-tank strategies
larviculture
Embryo Handling and Care
Larval Biology
Feeding
Water Quality
housing density
genetic management
identification and record keeping
Identification
Tank labels
Record Keeping
transportation
Shipping
General physical requirements
Sample packaging method – adults
Sample packaging method – embryos
Receiving
General physical requirements
Biosecurity concerns
Sample receiving method – adults
Sample receiving method – embryos
Regulatory Requirements

3 Life support
facility design
Water Handling and Resistance
Water Production
Infrastructure
Electricity
Environmental Controls
Alarming
Access
Work Zones
Storage
Rack Configuration
Quarantine
Air
Sinks
Cleaning and Sterilization
Redundancy
housing systems
Tanks
Racks
Water Treatment
water source
Testing and Analysis
Treatment Methods
Reverse osmosis
Deionization
Combined water treatment systems
Treatment System Maintenance
Water Conditioning
water quality management
Key Parameters
Temperature
pH
Alkalinity
Hardness
Salinity
Dissolved oxygen
Carbon dioxide
Nitrogenous wastes
Testing
effluent management

4 Management
regulatory agencies and compliance
institutional animal care and use committees
Procurement and the Source of Animals
occupational health and zoonotic diseases
disposal of fish

5 Veterinary care
basic biologic parameters
animal welfare indicators for fish
Change in Color
Respiratory Rate
Swimming and Behavioral Patterns
Growth Rate
Body Condition
External Morphological Abnormalities
Reproductive Performance
Biochemical Welfare Indicators
Food Consumption
stress response in fish
clinical case management and sample collection
Source of the Fish
Management Practices
Genetic Background
Age of the Fish
Research Application
Water
Physical Examination
Simple protocol for preparing cutaneous wet
mount preparations
Gill wet mount preparation
Skin mucus wet mount preparation
Fin wet mount preparation
Necropsy
common diseases of the laboratory zebrafish
Bacterial Agents and Disease
Bacterial gill disease
Flavobacterium columnare
Aeromonas hydrophil
Mycobacteriosis
Parasitic Agents
Pseudocapillaria tomentosa
Myxosporidiosis
Metazoan Parasites
Microsporidiosis
Pleistophora hyphessobryconis
Fungal Diseases
Saprolegniasis
Lecythophora mutabilis
Parasitic Diseases
Viruses
Endogenous retrovirus in zebrafish
Non-Infectious Conditions
Ammonia toxicity
Nitrite toxicity
Chlorine/chloramine toxicity
Gas bubble disease
preventive medicine
Zebrafish Diets
Source of Fish
Disinfection of Equipment and Cleanliness
health surveillance and monitoring
anesthesia and euthanasia
Chemical Agents
Cooling
Bleach

6 Experimental methodology
restraint
Handling
Basic Handling Principles
Protection of the mucus layer
Minimizing light, noise, and vibration
Anesthesia and sedation
Other general considerations
Methods of Restraint
Adults and juveniles
Larval fish
Fasting
sampling methods
Tissue Biopsy
Urine
Blood
compound administration
Branchial Diffusion
Oral Dosing
Injection
surgical procedures
Surgical Preparation
Instrumentation
Wound Closure and Wound Healing
Anesthesia
Postoperative Care
Research Endpoints

7 Resources
organizations
publications
Books
Periodicals
electronic resources
equipment and supplies

Includes bibliographical references (p. 199-233) and index(p. 239-254)