Principles and techniques of practical biochemistry / edited by Keith Wilson and John M. Walker.

CONTENT

1 GENERAL PRINCIPLE OF BIOCHEMICAL INVESTIGATIONS
1.1 Introduction
1.2 General approaches to biochemical investigations
1.3 Physiological
1.4 Cell distruption
1.5 Cell and tissue culture, etc.

2 IMMUNOCHEMICAL TECHNIQUES
2.1 General Principles
2.2 Production of antibodies
2.3 The Precipitin reaction in free solution
2.4 The precipitation reaction in gels: immunodiffusion{ ID}
2.5 Radioimmunoassay{ RIA}, ETC.

3 MOLECULAR BIOLOGY TECHNIQUES
3.1 Introduction
3.2 Structure of nucleic acids
3.3 Functions of nucleic acids
3.4 Isolation of nucleic acids
3.5 Physical analysis of DNA, etc.

4 PROTEIN AND ENZYME TECHNIQUES
4.1 Ionic properties of amino acids and proteins
4.2 Protein structure
4.3 Investigation of protein structure
4.4 Total protein estimation
4.5 Protein purification, etc.

5 RADIOISOTOPE TECHNIQUES
5.1 The nature of radioactivity
5.2 Detection and measurement of radioactivity
5.3 Other practical aspects of counting radio labelled compounds
5.4 Inherent advantages and restrictions of radio tracer experiments
5.5 Safety aspects, etc.

6 CENTRIFUGATION TECHNIQUES
6.1 Introduction
6.2 Basic principle of sedimentation
6.3 Centrifuges and their use
6.4 Design and care of preparative rotors
6.5 Sample containers, etc.

7 SPECTROSCOPIC TECHNIQUES
7.1 Introduction
7.2 Gamma-ray spectroscopy and y-ray resonance spectroscopy
7.3 X-ray spectroscopy
7.4 Ultraviolet and visible light spectroscopy
7.5 Infrared and Raman spectroscopy, etc.

8 MASS SPECTROMETRIC TECHNIQUES
8.1 Introduction
8.2 The mass spectrometer
8.3 Electron impact ioniSation
8.4 Chemical ioniSation {CI}
8.5 Field ionisation {FI}, etc.

9 ELECTROPHORETIC TECHNIQUES
9.1 General principles
9.2 Support media
9.3 Electrophoresis of proteins
9.4 Electrophoresis of nucleic acids
9.5 Capillary electrophoresis, etc.

10 CHROMATOGRAPHIC TECHNIQUES
10.1 General principle
10.2 Sample preparation
10.3 Low pressure Column Chromatography
10.4 High performance liquid chromatography {HPLC}
10.5 Adsorption Chromatography, etc.

11 ELECTROCHEMICAL TECHNIQUES
11.1 Introduction
11.2 Principles of electrochemical techniques
11.3 Redox reactions
11.4 The PH electrode
11.5 Ion-selective and gas-sensing electrodes, erc.

Includes bibliographical index: p. 575-586