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Flow cytometry : a practical approach / edited by Michael G. Ormerod.

Contributor(s): Series: The practical approach series ; 229Publication details: Oxford [England] ; New York : Oxford University Press, 2000.Edition: 3rd editionDescription: xx, 276 p. : ill. ; 26 cmISBN:
  • 019963825X (hbk.)
  • 0199638241 (pbk.)
Subject(s): DDC classification:
  • QY 95 F 644 2000 F 644 2000
Online resources:
Contents:
Contents 1. Introduction to the principles of flow cytometry, Nigel P. Carter and Michael G. Ormerod 2. Fluorescence and fluorochromes, Michael G. Ormerod 3. Preparing suspensions of single cells, Michael G. Ormerod 4. Flow sorting, Nigel P. Carter and Michael G. Ormerod 5. Immunofluorescence of surface markers, Michael R. Loken, Cherie I. Green, and Denise A. Wells 6. Analysis of DNA - general methods, Michael G. Ormerod 7. Further clinical applications, Terry Hoy, et al. 8. Quality assurance in the clinical laboratory, Jan W. Gratama and David Barnett 9. Measurement of cytoplasmic and nuclear antigens, Jorgen K. Larsen 10. Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method, George D. Wilson 11. Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method, Michael G. Ormerod and Martin Poot 12. Chromosome analysis and sorting by flow cytometry, Derek C. Davies, Simon P. Monard, and Bryan D. Young 13. Intracellular ionised calcium, magnesium, membrane potential and pH, Peter S. Rabinovitch and Carl H. June 14. Flow cytometry in the study of apoptosis, Michael G. Ormerod 15. Further applications to cell biology, Michael G. Ormerod A1. Safety Procedures A2. Flow cytometers and software A3. List of suppliers
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Includes bibliographical references and index.

Contents

1. Introduction to the principles of flow cytometry, Nigel P. Carter and Michael G. Ormerod
2. Fluorescence and fluorochromes, Michael G. Ormerod
3. Preparing suspensions of single cells, Michael G. Ormerod
4. Flow sorting, Nigel P. Carter and Michael G. Ormerod
5. Immunofluorescence of surface markers, Michael R. Loken, Cherie I. Green, and Denise A. Wells
6. Analysis of DNA - general methods, Michael G. Ormerod
7. Further clinical applications, Terry Hoy, et al.
8. Quality assurance in the clinical laboratory, Jan W. Gratama and David Barnett
9. Measurement of cytoplasmic and nuclear antigens, Jorgen K. Larsen
10. Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method, George D. Wilson
11. Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method, Michael G. Ormerod and Martin Poot
12. Chromosome analysis and sorting by flow cytometry, Derek C. Davies, Simon P. Monard, and Bryan D. Young
13. Intracellular ionised calcium, magnesium, membrane potential and pH, Peter S. Rabinovitch and Carl H. June
14. Flow cytometry in the study of apoptosis, Michael G. Ormerod
15. Further applications to cell biology, Michael G. Ormerod
A1. Safety Procedures
A2. Flow cytometers and software
A3. List of suppliers

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