Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual / Dan Burke, Dean Dawson, Tim Stearns.
Publication details: Plainview, N.Y. : Cold Spring Harbor Laboratory Press, c2000.Edition: 2000 editionDescription: xvii, 205 p. : ill. ; 28 cmISBN:- 0879695889
- Methods in yeast genetics.
- QW 568 C997 2000
Item type | Current library | Call number | Copy number | Status | Date due | Barcode |
---|---|---|---|---|---|---|
Book Open Access | Health Sciences Library | QW 568 C997 2000 (Browse shelf(Opens below)) | 1 | Available | MBAL22010982 |
Includes bibliographical references.
Contents
Introduction
Genetic Nomenclature
Experiments
I. Looking at yeast cells
II. Isolation and characterization of auxotropic temperature-sensitive, and UV-sensitive mutants
III. Meiotic mapping
IV. Meiotic Recombination and Random Spore Analysis
V. Transformation of yeast
VI. Synthetic lethal mutants
VII. Gene replacement
VIII. Isolation of ras2 suppressors
IX. Manipulating cell types
X. Isolating mutants by insertional mutagenesis
XI. Two-hybrid protein interaction method
Techniques and protocols
1. High-efficiency transformation of yeast
2. "Lazy bones" plasmid transformation of yeast colonies
3. Yeast DNA isolations
4. Yeast protein extracts
5. Yeast RNA isolation
6. Hdroxylamine mutagenesis of plasmid DNA
7. Assay of B-galactosidase in yeast
8. Plate assay for carboxypeptidase Y
9. Random spore analysis
10. Yeast vital stains
11. Yeast immunofluorescence
12. Actin staining in fixed cells
13. PCR protocol for PCR-mediated gene disruption
14. Yeast colony PCR protocol
15. Measuring yeast cell density by spectrophotometry
16. Cell synchrony
17. Chromatin immunoprecipitation
18. Flow cytometry of yeast DNA
19. Logarithmic growth
20. EMS mutagenesis
21. Tetrad dissection
22. Making a tetrad dissection needle
23. Picking zygotes
24. Determining plating efficiency
Appendices.
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