Gene and chromosome analysis, part B : methods in molecular genetics / edited by Kenneth W. Adolph.

Contents;

Section I: Recombinant DNA
1. Isolation of cDNA clones of low-abundance mRNAs: Alternative strategies in different in differential and subtractive hybridization
2. Random sequence selection for generation of active recombinant molecules
3. Improved vectors for expression and purification of recombinant proteins

Section II: Chromosome and and DNA analysis
4. Synaptic complexes and RecA-Assisted restriction endonuclease cleavage of DNA
5. Retroviral gene transfer vectors containing single cleavage sites for mapping mammalian genomes
6. Extrachromosomal elements in mammalian cells
7. p53 gene mutations in human premalignant and malignant tissues
8. Junction mapping of translocation chromosomes by fluorescence in situ hybridization and computer image analysis in human solid tumors.
9. Analysis of Megabase DNA using pulsed field gel electrophoresis techniques.

Section III: Immunoglobulin genes
10. Molecular analysis of antibody diversity
11. Production and expression of genetically engineered immunoglobulins
12. Physical mapping of variable region gene locus of human immunoglobulin heavy chain
13. Application of immunoglobulin chimeras to receptor - Ligand studies

Section IV: Mutants and mutagenic activity
14. Conditional mutants for studying functions of calmodulin in
15. Heritable mutagenic activity in maize inbred line A188

Section V: Replication, transcription and translation
16. Fidelity of DNA replication in human cells
17. Transcription factor interactions with model nucleosomal templates
18. Technology to study gene transcription in normal T-Lymphoctye clones
19. Primer extension analysis of RNA
20. Translational control by polyadenylate elongation during early development

Index : p. 363-369.