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Benson's microbiological applications : laboratory manual in general microbiology / Alfred E. Brown

By: Publication details: Boston : McGraw-Hill, c2007Edition: 10th edition, Short VersionDescription: xiv, 450 p. : col. ill. ; 28 cmISBN:
  • 9780072992724
Subject(s): DDC classification:
  • 22 579.078 BRO
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Content

Part 1Microscopy
1 Brightfield Microscopy
2 Darkfield Microscopy
3 Phase-Contrast Microscopy
4 Microscopic Measurements


Part 2 Survey of Microorganisms
5 Protozoans, Algae, and Cyanobacteria
6 Ubiquity of Bacteria
7 The Fungi: Yeasts and Molds


Part 3 Manipulation of Microorganisms
8 Aseptic Technique
9 Pure Culture Techniques


Part 4 Staining and Observation of Microorganisms
10 Smear Preparation
11 Simple Staining
12 Negative Staining
13 Capsular Staining
14 Gram Staining
15 Spore Staining: Two Methods
16 Acid-Fast Staining: Ziehl-Neelsen Method
17 Motility Determination


Part 5 Culture Methods
18 Culture Media Preparation
19 Cultivation of Anaerobes
20 Enumeration of Bacteria: The Standard Plate Count
21 Slime Mold Culture
22 Slide Culture: Molds


Part 6 Bacterial Viruses
23 Determination of a Bacteriophage Titer
24 Isolation of Phage from Flies
25 Phage Typing


Part 7 Environmental Influences and Control of Microbial Growth
26 Temperature: Effects on Growth
27 Temperature: Lethal Effects
28 pH and Microbial Growth
29 Water Activity and Osmotic Pressure
30 Ultraviolet Light: Lethal Effects
31 Oligodynamic Action
32 Evaluation of Alcohol: Its Effectiveness as an Antiseptic
33 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
34 Evaluation of Antiseptics: The Filter Paper Disk Method
35 Effectiveness of Hand Scrubbing


Part 8 Identification of Unknown Bacteria
36 Morphological Study of Unknown Bacterium
37 Cultural Characteristics
38 Physiological Characteristics: Oxidation and Fermentation Tests
39 Physiological Characteristics: Hydrolytic Reactions
40 Physiological Characteristics: Biochemical Tests
41 Use of Bergey's Manual


Part 9 Miniaturized Multitest Systems
42 Enterobacteriaceae Identification: The API 20E System
43 Enterobacteriaceae Identification: The Enterotube II System
44 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System
45 Staphylococcus Identification: The API Staph-Ident System


Part 10 Applied Microbiology
46 Bacterial Counts of Foods
47 Bacterial Examination of Water: Qualitative Tests
48 The Membrane Filter Method
49 Reductase Test
50 Microbial Spoilage of Canned Food
51 Microbiology of Alcohol Fermentation


Part 11 Medical Microbiology and Immunology
52 The Staphylococci: Isolation and Identification
53 The Streptococci: Isolation and Identification
54 Gram Negative Intestinal Pathogens
55 Slide Agglutination Test: Serological Typing
56 Slide Agglutination (Latex) Test: For S. aureus Identification
57 Tube Agglutination Test: The Heterophile Antibody Test
58 White Blood Cell Study: The Differential WBC Count
59 Blood Grouping
60 A Synthetic Epidemic
Appendixes
A Tables
B Indicators, Stains, Reagents
C Media
D Identification Charts
E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics

Includes Bibliographic References(p. 437 - 438) and Index(p.439 - 450)

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