Recombinant DNA and biotechnology : a guide for students / Helen Kreuzer, Adrianne Massey.

Includes bibliographical references and index.

CONTENTS

PART 1
Laying the Foundation 1
1 An overview of Biotechnology 3
2 Biotechnology and Society 33
3 Genes, Genetics and Geneticists 53
4 An overview of molecular Biology 75
5 Applying Molecular Biology:
Recombinant DNA Technology 115

PART II
Classroom Activities 141
A. DNA Structure and Function 143
6 Constructing a paper Helix 144
7 DNA polymerase: The replicator 146
8 From Genes to Proteins 148 . etc.

B. Manipulation and Analysis of DNA 159
11 DNA Scissors 160
12 DNA Goes to the Races 162
13 Restriction Analysis of Lambda DNA 166
14 Recombinant paper plasmids 171
15 Restriction Analysis challenge 174 .etc.

C. Transfer of Genetic Information 207
23 Transformation of Escherichia coli 208
24 Conjugative Transfer of Antibiotic Resistance in Escherichia coli 211
Reading: Gene Transfer in Nature 214
25 transduction of an Antibiotic Resistance Gene 219. etc.

D. Bioinformatics and Evolutionary Analysis of Proteins
27 Testing for Amylase Activity 228
28 Electrophoresis of Amylase Samples 233. etc.

E. Molecular Biology and Genetics 247
31 An adventure in Dog Hair, Part I 248
32 An adventure in Dog Hair, Part II: yellow Labs 252. etc

PART III
Societal Issues 273
Introduction 275
34 Science, Technology and Society 277
35 Weighing Technology's Risks and Benefits 280 . etc.
Reading: Are Bioengineered Foods Safe? 293

Appendix A: Templates 321
Glossary 411
Index 419