Experimental biochemistry / Robert L. Switzer, Liam F. Garrity.

I. BASIC TECHNIQUES OF EXPERIMENTAL BIOCHEMISTRY
Introduction
Requirements for a Student of Experimental Biochemistry
Units of Biochemistry
Analysis and Interpretation of Experimental Data
Presentation of Experimental Data
To the Instructor
Expt. 1 Photometry/Spectroscopy
Expt. 2 Chromatography
Expt. 3 Radioisotope Techniques
Expt. 4 Electrophoresis

II. PROTEINS AND ENZYMOLOGY
Introduction
Amino Acids: Identification and Quantitative Determination
Amino Acids: Ionic Properties
Protein Structure
Determination of Protein Structure
Determination of Molecular Weights and Quaternary Structures of Protein Denaturation
Protein Purification
Quantitative Determination of Proteins
Enzymology
Kinetics of Inhibition of Enzymes
Other Factors Affecting Enzyme Activity
Enzyme Assay
Assay Procedure During Enzyme Purification
Expt. 5 Acid-Base Properties of Amino Acids
Expt. 6 Sequence Determination of a Dipeptide
Expt. 7 Study of the Properties of b-Galactosidase
Expt. 8 Purification of Glutamate-Oxaloacetate Transaminase from Pig Heart
Expt. 9 Kinetic and Regulatory Properties of Aspartate Transcarbamylase
Expt. 10 Affinity Purification of Glutathione-S-Transferase—NEW!

III. BIOMOLECULES AND BIOLOGICAL SYSTEMS
Introduction
Carbohydrates
Lipids
Membranes
Expt. 11 Microanalysis of Carbohydrate Mixtures by Isotopic, Enzymatic and Colorimetric Methods
Expt. 12 Glucose-1-Phosphate: Enzymatic Formation from Starch and Chemical Characterization
Expt. 13 Isolation and Characterization of Erythrocyte Membranes
Expt. 14 Electron Transport
Expt. 15 Study of the Phosphoryl Group Transfer Cascade Governing Glucose Metabolism: Allosteric and Covalent Regulation of Enzyme Activity
Expt. 16 Experiments in Clinical Biochemistry and Metabolism

IV. IMMUNOCHEMISTRY
Introduction
Types of Antibodies
Polyclonal and Monoclonal Antibodies
Purification of Antibodies
Use of Anitbodies in Biochemical Studies
Advantages and Disadvantages of Polyclonal and Monoclonal Antibodies
Expt. 17 Partial Purification of a Polyclonal Antibody, Determination of Titer, and Quantitation of an Antigen Using the ELISA
Expt 18. Western Blot to Identify an Antigen

V. NUCLEIC ACIDS
Introduction
Structural Features of Nucleic Acids
Cellular Localization and Roles of Nucleic Acids
Chemical Properties of Nucleic Acids
Physical Properties of Nucleic Acids
Plasmids and Other Cloning Vectors
Restriction Endonucleases and Other Modifying Enzymes
Ligation of DNA Fragments
Transformation of Host Cells
Selection and Screening for Transformed Cells
Expt. 19 Isolation of Bacterial DNA
Expt. 20 Transformation of a Genetic Character
Expt. 21 Constructing and Characterizing a Recombinant DNA Plasmid
Expt. 22 In Vitro Transcription from a Plasmid Carrying a T7 RNA Polymerase Specific Promoter
Expt. 23 In Vitro Translation: mRNA, tRNA, and Ribosomes
Expt. 24 Amplification of a DNA Fragment Using Polymerase Chain Reaction

VI. INFORMATION SCIENCE
Introduction
Specialized Databases
Computer Software Programs for Analyzing Sequences
The Protein Data Bank (PDB) and RasMol
Expt. 25 Obtaining and Analyzing Genetic and Protein Sequence Information via the World Wide Web, Lasergene, and RasMol

Includes bibliographical references and index.