MARC details
| 000 -LEADER |
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| fixed length control field |
03663cam a22003014a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20230922072806.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
041203s2005 nyua b 001 0 eng |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
0879697288 (pbk. : alk. paper) |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
BUL |
| Transcribing agency |
BUL |
| Modifying agency |
BUL |
| Language of cataloging |
eng |
| Description conventions |
rda |
| 060 10 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
|---|
| Classification number |
QW 25 A491m 2005 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Amberg, David C. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Methods in yeast genetics : |
| Remainder of title |
a Cold Spring Harbor Laboratory course manual / |
| Statement of responsibility, etc. |
David C. Amberg, Daniel Burke, Jeffrey Strathern. |
| 250 ## - EDITION STATEMENT |
|---|
| Edition statement |
2005 edition |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cold Spring Harbor, N.Y. : |
| Name of publisher, distributor, etc. |
Cold Spring Harbor Laboratory Press, |
| Date of publication, distribution, etc. |
c2005. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xvii, 230 p. : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
28 cm. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references and index. |
| 505 ## - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Contents<br/><br/>Introduction <br/><br/>Genetic Nomenclature<br/> <br/>Experiments<br/>I. Looking at Yeast Cells <br/>II. Isolation and Characterization of Auxotrophic, Temperature-<br/> sensitive, and Osmotic-sensitive Mutants <br/>III. Meiotic Mapping <br/>IV. Mitotic Recombination and Random Spore Analysis <br/>V. Transformation of Yeast <br/>VI. Synthetic Lethal Mutants <br/>VII. Gene Replacement <br/>VIII. Isolation of ras2 Suppressors <br/>IX. Manipulating Cell Types <br/>X. Isolating Mutants by Insertional Mutagenesis <br/>XI. Isolation of Separation of Function Mutants by Two-hybrid Differential Interaction Screening <br/><br/>Techniques and Protocols<br/>1. High-efficiency Transformation of Yeast <br/>2. Quick and Dirty Plasmid Transformation of Yeast Colonies <br/>3. Yeast DNA Isolations <br/> A. Yeast DNA Miniprep (40 ml) <br/> B. Yeast DNA Miniprep (5 ml) <br/> C. A Ten-minute DNA Preparation from Yeast <br/>D. Yeast Genomic DNA: Glass Bead Preparation<br/>E. Yeast Genomic DNA by Spheroplasting <br/>4. Yeast Protein Extracts <br/>5. TAP Tag Protein Purification<br/>6. Yeast RNA Isolation <br/>7. Hydroxylamine Mutagenesis of Plasmid DNA <br/>8. Assay of ยง-Galactosidase in Yeast <br/>9. Plate Assay for Carboxypeptidase Y <br/>10. Random Spore Analysis <br/>11. Yeast Vital Stains <br/>A. DAPI Stain of Nuclear and Mitochondrial DNA<br/>B. Visualizing Mitochondria with DiOC6 or DiIC5(3)<br/>C. Visualizing Vacuoles and Endocytic Compartments with FM4-64. <br/>D. Calcofluor Staining of Chitin and Bud Scars<br/>12. Yeast Immunofluorescence <br/>13. Actin Staining in Fixed Cells <br/>14. PCR-Mediated Gene Disruption<br/>A. One-Step PCR Gene Disruption.<br/>B. Gene Disruption by Double-Fusion PCR <br/>15. Yeast Colony PCR <br/>16. Measuring Yeast Cell Density by Spectrophotometry <br/>17. Cell Synchrony <br/>18. Chromatin Immunoprecipitation <br/>19. Flow Cytometry of Yeast DNA <br/>20. Logarithmic Growth <br/>21. EMS Mutagenesis <br/>22. Tetrad Dissection <br/>23. Making a Tetrad Dissection Needle <br/>24. Picking Zygotes <br/>25. Determining Plating Efficiency<br/>26. DNA Mini-Prep from E. coli <br/>27. Preparing and Transforming Competent E. coli<br/>28. Storing and handling of the systematic deletion collection.<br/>Appendices<br/> A. Media <br/> B. Stock Preservation <br/> C. Yeast Genetic and Physical Maps <br/> D. Templates for Making Streak Plates <br/> E. Electrophoretic Karyotypes of Strains for Southern Blot Mapping <br/> F. Strains <br/> G. Counting Yeast Cells with a Standard Hemocytometer Chamber <br/> H. Tetrad Scoring Sheet <br/> I. Trademarks |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Yeast fungi |
| General subdivision |
Genetics |
| Form subdivision |
Laboratory manuals. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Yeasts |
| General subdivision |
genetics |
| Form subdivision |
Laboratory Manuals. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Burke, Dan, |
| Dates associated with a name |
1954- |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Strathern, Jeffrey N. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Burke, Dan, |
| Dates associated with a name |
1954- |
| Title of a work |
Methods in yeast genetics. |
| 710 2# - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Cold Spring Harbor Laboratory. |
| 856 41 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Materials specified |
Table of contents |
| Uniform Resource Identifier |
<a href="http://www.loc.gov/catdir/toc/ecip055/2004028765.html">http://www.loc.gov/catdir/toc/ecip055/2004028765.html</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Book Reference |
| Edition |
2005 edition |
| Classification part |
QW 25 A491m 2005 |
| Item part |
1 |
| Call number prefix |
QW 25 |
| Call number suffix |
A491m 2005 |
