TY  - BOOK
AU  - Trigiano,R.N.
AU  - Gray,Dennis J.
TI  - Plant tissue culture, development and biotechnology
SN  - 9781420083262 (pbk. : alk. paper)
U1  - 571.538 22
PY  - 2011///
CY  - Boca Raton, FL
PB  - CRC Press
KW  - Plant tissue culture
KW  - Laboratory manuals
N1  - CONTENTS

Section I Introduction
Chapter 1. Introduction

Section II Supporting Methodologies and General Concepts
Chapter 2. Getting Started with Tissue Culture—Media Preparation, Sterile Technique,
and Laboratory Equipment.

Chapter 3. Nutrition of Cell and Organ Cultures

Chapter 4. PGRs and Their Use in Micropropagation

Chapter 5. Elements of In Vitro Research

Chapter 6. Proper Use of Microscopes

Chapter 7. Plant Histological Techniques

Chapter 8. A Brief Introduction to Plant Anatomy and Morphology

Chapter 9. Seed Development and Germination

Chapter 10. Molecular Tools for Studying Plant Genetic Diversity

Chapter 11. Molecular Approaches to the Study of Plant Development

Section III Propagation and Development Concepts
Chapter 12. Propagation by Shoot Culture 

Chapter 13. Micropropagation of Syngonium by Shoot Culture

Chapter 14. Micropropagation and In Vitro Flowering of Rose

Chapter 15. Micropropagation of Potato by Node Culture and Microtuber Production

Chapter 16. Commercial Laboratory Production

Chapter 17. Detection and Elimination of Microbial Endophytes and Prevention
of Contamination in Plant Tissue Culture

Chapter 18. Culture Indexing for Bacterial and Fungal Contaminants

Chapter 19. Propagation from Nonmeristematic Tissues—Organogenesis

Chapter 20. Developing a Molecular Understanding of In Vitro and In Planta
Shoot Organogenesis

Chapter 21. Direct Shoot Organogenesis from Leaf Explants of Chrysanthemum
and African Violets

Chapter 22. Propagation from Nonmeristematic Tissues—Nonzygotic Embryogenesis

Chapter 23. Developmental and Molecular Aspects of Nonzygotic (Somatic)
Embryogenesis

Chapter 24. Embryogenic Callus and Suspension Cultures from Leaves of Orchardgrass
Chapter 25. Direct Nonzygotic Embryogenesis from Leaves and Flower Receptacles
of Cineraria

Section IV Crop Improvement Techniques
Chapter 26. Protoplasts—An Increasingly Valuable Tool in Plant Research

Chapter 27. Demonstration of Principles of Protoplast Isolation Using Chrysanthemum
and Orchardgrass Leaves

Chapter 28. Isolation, Culture, and Fusion of Tobacco and Potato Protoplasts

Chapter 29. Haploid Cultures

Chapter 30. Production of Haploid Tobacco and Potato Plants Using Anther Culture

Chapter 31. Embryo Rescue

Chapter 32. Promoters and Gene Expression Regulation

Chapter 33. Genetic Engineering Technologies

Chapter 34. Transformation of Plant Meristems

Chapter 35. Genetic Transformation of Chysanthemum and Tobacco Using
Agrobacterium tumefaciens

Chapter 36. Genetic Transformation of Tobacco and Production of Transgenic Plants

Chapter 37. Genetically Modified Controversies

Chapter 38. Cryopreservation of Plant Cells, Tissues, and Organs

Chapter 39. Cryopreservation of In Vitro Grown Shoot Tips

Chapter 40. Cryopreservation of Orthodox and Recalcitrant Seed

Chapter 41. Plant Biotechnology for the Production of Natural Products

Chapter 42. Pigment Production in Ajuga Cell Cultures

Chapter 43. Variation in Tissue Culture

Section V The Business of Biotechnology

Chapter 44. Biotechnology Entrepreneurship in the 21st Century

Chapter 45. Intellectual Property Protection for
; Includes bibliographical references and index
ER  -