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Eukaryotic transcription factors / David S. Latchman.

By: Publication details: Amsterdam ; Boston : Elsevier/Academic Press, 2004Edition: 4th editionDescription: xxvi, 360 p. : ill. ; 26 cmISBN:
  • 0124371787 (alk. paper)
Subject(s): DDC classification:
  • QU 300 L35ie 2003 L35ie 2003
Online resources:
Contents:
Content DNA sequences, transcription factors and chromatin structure The importance of transcription Chromatin structure and its remodelling Chromatin structure and gene regulation Chromatin remodelling factors Histone acetylation DNA sequence elements The gene promoter Sequences involved in the basic process of transcription Sequences involved in regulated transcription Sequences which act at a distance Negatively acting DNA sequences Interaction between factors bound at various sites Conclusions References Methods for studying transcription factors Introduction Methods for studying DNA-protein interactions DNA mobility shift assay DNaseI footprinting assay Methylation interference assay In vivo Footprinting assay Methods for purifying and/or cloning transcription factors Protein purification Gene cloning Use of cloned genes Domain mapping of transcription factors Determining the DNA binding specificity of an uncharacterised factor Identification of target genes for transcription factors Conclusions References Transcription factors and constitutive transcription RNA polymerases The stable transcriptional complex RNA polymerase I RNA polymerase III RNA polymerase II Stepwise assembly of the RNA polymerase II basal transcriptional complex The RNA polymerase holoenzyme TBP, The universal transcription factor? Conclusions References Families of DNA binding transcription factors Introduction The homeodomain Transcription factors in Drosophila development The homeobox DNA binding by the helix-turn-helix motif in the homeobox Regulation of DNA binding specificity by interaction between different homeobox proteins Homeodomain transcription factors in other organisms POU proteins Pax proteins The two cysteine two histidine finger Transcription factors with the two cysteine two histidine finger DNA binding by the two cysteine two histidine finger The multi-cysteine finger Steroid receptors DNA binding by the multi-cysteine zinc finger The basic DNA binding domain The leucine zipper and the basic DNA binding domain The helix-loop-helix motif and the basic DNA binding domain Dimerisation of basic DNA binding domain-containing factors Other DNA-binding motifs Conclusions References Activation of gene expression by transcription factors Activation domains Nature of activation domains Acidic domains Glutamine-rich domains Proline-rich domains Functional relationship of the different activation domains Interaction of activation domains with the basal transcriptional complex Activators and the basal transcriptional complex Stimulation of factor binding Stimulation of factor activity Interaction of activation domains with other regulatory proteins The mediator complex TAFs CBP and other co-activators A multitude of targets for transcriptional activators Other targets for transcriptional activators Modulation of chromatin structure Stimulation of transcriptional elongation Conclusions Repression of gene expression by transcription factors Repression of transcription Indirect repression Inhibition of activator binding by masking of its DNA binding site Inhibition of activator binding by formation of a non-DNA binding complex Quenching of an activator Degradation of an activator Direct repression Mechanisms of transcriptional repression Direct repression by DNA binding transcription factors Direct repression by factors binding to the basal transcriptional complex Other targets for transcriptional repressors Modulation of chromatin structure Inhibition of transcriptional elongation Conclusions References Regulation of transcription factor synthesis Transcription factor regulation Regulated synthesis of transcription factors The MyoD transcription factor Homeobox transcription factors Mechanisms regulating the synthesis of transcription factors Regulation of transcription Regulation of RNA splicing Regulation of translation Conclusions Regulation of transcription factor Evidence for the regulated activity of transcription factors Regulation by protein-ligand binding Examples of regulation by ligand binding The nuclear receptors Regulation by protein-protein interactions Inhibition of transcription factor activity by protein-protein interaction Activation of transcription factors by protein-protein interaction Alteration of transcription factor function by protein-protein interaction Regulation by protein modification Transcription factor modification Phosphorylation Acetylation Methylation Ubiquitination Regulation by protein degradation and processing Role of regulated activity Conclusions Transcription factors and human disease Diseases caused by transcription factor mutations Cancer Cellular oncogenes and cancer Fos, Jun and AP1 v-erbA and the thyroid hormone receptor The myc oncogene Other oncogenic transcription factors Anti-oncogenes and cancer Nature of anti-oncogenes p53 The Retinoblastoma protein Other anti-oncogenic transcription factors. Conclusions References Conclusions and future prospects
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Book Open Access Book Open Access Health Sciences Library QU 300 L351e 2003 (Browse shelf(Opens below)) 1 Available MBAL22010942

Includes bibliographical references and index.

Content
DNA sequences, transcription factors and chromatin structure
The importance of transcription
Chromatin structure and its remodelling
Chromatin structure and gene regulation
Chromatin remodelling factors
Histone acetylation
DNA sequence elements
The gene promoter
Sequences involved in the basic process of transcription
Sequences involved in regulated transcription
Sequences which act at a distance
Negatively acting DNA sequences
Interaction between factors bound at various sites
Conclusions
References
Methods for studying transcription factors
Introduction
Methods for studying DNA-protein interactions
DNA mobility shift assay
DNaseI footprinting assay
Methylation interference assay
In vivo Footprinting assay
Methods for purifying and/or cloning transcription factors
Protein purification
Gene cloning
Use of cloned genes
Domain mapping of transcription factors
Determining the DNA binding specificity of an uncharacterised factor
Identification of target genes for transcription factors
Conclusions
References
Transcription factors and constitutive transcription
RNA polymerases
The stable transcriptional complex
RNA polymerase I
RNA polymerase III
RNA polymerase II
Stepwise assembly of the RNA polymerase II basal transcriptional
complex
The RNA polymerase holoenzyme
TBP, The universal transcription factor?
Conclusions
References
Families of DNA binding transcription factors
Introduction
The homeodomain
Transcription factors in Drosophila development
The homeobox
DNA binding by the helix-turn-helix motif in the homeobox
Regulation of DNA binding specificity by interaction between different
homeobox proteins
Homeodomain transcription factors in other organisms
POU proteins
Pax proteins
The two cysteine two histidine finger
Transcription factors with the two cysteine two histidine finger
DNA binding by the two cysteine two histidine finger
The multi-cysteine finger
Steroid receptors
DNA binding by the multi-cysteine zinc finger
The basic DNA binding domain
The leucine zipper and the basic DNA binding domain
The helix-loop-helix motif and the basic DNA binding domain
Dimerisation of basic DNA binding domain-containing factors
Other DNA-binding motifs
Conclusions
References
Activation of gene expression by transcription factors
Activation domains
Nature of activation domains
Acidic domains
Glutamine-rich domains
Proline-rich domains
Functional relationship of the different activation domains
Interaction of activation domains with the basal transcriptional complex
Activators and the basal transcriptional complex
Stimulation of factor binding
Stimulation of factor activity
Interaction of activation domains with other regulatory proteins
The mediator complex
TAFs
CBP and other co-activators
A multitude of targets for transcriptional activators
Other targets for transcriptional activators
Modulation of chromatin structure
Stimulation of transcriptional elongation
Conclusions
Repression of gene expression by transcription factors
Repression of transcription
Indirect repression
Inhibition of activator binding by masking of its DNA binding site
Inhibition of activator binding by formation of a non-DNA binding
complex
Quenching of an activator
Degradation of an activator
Direct repression
Mechanisms of transcriptional repression
Direct repression by DNA binding transcription factors
Direct repression by factors binding to the basal transcriptional complex
Other targets for transcriptional repressors
Modulation of chromatin structure
Inhibition of transcriptional elongation
Conclusions
References
Regulation of transcription factor synthesis
Transcription factor regulation
Regulated synthesis of transcription factors
The MyoD transcription factor
Homeobox transcription factors
Mechanisms regulating the synthesis of transcription factors
Regulation of transcription
Regulation of RNA splicing
Regulation of translation
Conclusions
Regulation of transcription factor
Evidence for the regulated activity of transcription factors
Regulation by protein-ligand binding
Examples of regulation by ligand binding
The nuclear receptors
Regulation by protein-protein interactions
Inhibition of transcription factor activity by protein-protein interaction
Activation of transcription factors by protein-protein interaction
Alteration of transcription factor function by protein-protein interaction
Regulation by protein modification
Transcription factor modification
Phosphorylation
Acetylation
Methylation
Ubiquitination
Regulation by protein degradation and processing
Role of regulated activity
Conclusions
Transcription factors and human disease
Diseases caused by transcription factor mutations
Cancer
Cellular oncogenes and cancer
Fos, Jun and AP1
v-erbA and the thyroid hormone receptor
The myc oncogene
Other oncogenic transcription factors
Anti-oncogenes and cancer
Nature of anti-oncogenes
p53
The Retinoblastoma protein
Other anti-oncogenic transcription factors.
Conclusions
References
Conclusions and future prospects

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